



1. Turn off aquarium lights.
a) Dim the lights in the room where the shipping box will
be opened. Never open the box in bright light - severe stress or trauma may result from sudden exposure to bright light.
b) Float the sealed bag in the aquarium for 15 minutes. Never open the shipping bag at this time. This step allows the water in the shipping bag to adjust slowly to the temperature in the aquarium, while maintaining a high level of dissolved oxygen
2. Carefully empty the contents of the bags (including the water) into the buckets, making sure not to expose sensitive invertebrates to the air. Depending on the amount of water in each bag, this may require tilting the bucket at a 45 degree angle to make sure the animals are fully submerged.
You may need a prop or wedge to help hold the bucket in this position until there is enough liquid in the bucket to put it back to a level position.
3. Using airline tubing, set up and run a siphon drip line from the main aquarium to each bucket. You’ll need separate airline tubing for each bucket used. Tie several loose knots in the
airline tubing, to regulate flow from the aquarium. It is also a good idea to secure the airline tubing in place with an airline holder.
4. Begin a siphon by sucking on the end of the airline tubing you'll be placing into each of the buckets. When water begins flowing through the tubing, adjust the drip (by tightening one of the knots) to a rate of about 2-4 drips per second.
5. When the water volume in the bucket doubles, discard half and begin the drip again until the volume doubles once more – about one hour.
6. At this point, the specimens can be transferred to the aquarium.